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№6(6) 2023

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DOI 10.37219/2528-8253-2023-6-54

Bezshapochny SB, Bondarenko RV
EFFECT OF PLATELET-RICH PLASMA AND QUERCETIN COMBINATION ON NASAL MUCOSAL DAMAGE
IN CHRONIC ATROPHIC RINITIS
Bezshapochny Sergey B
Poltava State Medical University
Professor of Department of Otorhinolaryngology with Ophthalmology
Doctor of Medical Sciences, Professor
Email: bezshapochny@ukr.net
ORCID ID: https://orcid.org/0000-0002-8726-2024
Scopus Author ID: 6603471609
Bondarenko Ruslan V
Poltava State Medical University
Department of Otorhinolaryngology with Ophthalmology
Postgraduate student
Email: motoman.110494@gmail.com
ORCID ID: https://orcid.org/0000-0002-8497-8123

Abstract

Actuality: Biological barriers play an important role in the functioning of the body, protecting it from external bacterial invasion, and preventing the access of immunocompetent cells to organs to which immunological tolerance has not been formed (brain, testicular tissue, lens of the eye, etc.). A change in the structure of the mucous membrane of the nose undoubtedly leads to a violation of its functioning. Atrophic rhinitis is a chronic pathological process characterized by the formation of thick dry crusts in the nasal cavity, which occurs as a result of progressive atrophy of the nasal mucosa and underlying bone.

The purpose of this work is to study the effect of the combination of plasma enriched with platelets (PRP) and quercetin on the content of markers of oxidative damage to biological polymers, the concentration of hydrogen sulfide and the intensity of collagenolysis in the nasal mucosa under conditions of chronic atrophic rhinitis.

Materials and methods: The study was conducted on 73 patients. Patients were divided into control (n=20) and experimental (n=53) groups. Patients from the experimental group were randomly divided into 2 subgroups: the standard therapy group, patients who received standard treatment according to the protocols for 28 days (n=29) and the PRP-therapy group with the use of quercetin, patients who received PRP injections and quercetin orally at a dose of 40 mg 3 times a day for 28 days against the background of standard therapy for chronic atrophic rhinitis (n=24). The content of free L-oxyproline, the concentration of malondialdehyde, the concentration of hydrogen sulfide (H2S), and the content of oxidatively modified proteins were studied in the nasal mucosa.

Results: The use of PRP therapy and quercetin in combination with standard therapy of chronic atrophic rhinitis led to a decrease in the content of malondialdehyde in the nasal mucosa by 57.75%, the content of oxidatively modified proteins after treatment decreased by 55.05% compared to the indicators before treatment. The concentration of free L-oxyproline in the nasal mucosa after usage of PRP therapy and quercetin in combination with standard therapy of chronic atrophic rhinitis was reduced by 53.02% compared to the values before treatment. The content of H2S in the nasal mucosa dropped by 61.02% compared to the values before treatment.

Conclusions: The use of a medical complex, which against the background of standard therapy of chronic atrophic rhinitis includes the use of PRP therapy and quercetin, increases the effectiveness of the treatment by reducing oxidative damage to lipids and proteins, reducing the breakdown of collagen and limiting the accumulation of H2S in the nasal mucosa.

Keywords: platelet-rich plasma, atrophic rhinitis, lipid peroxidation, L-oxyproline, hydrogen sulfide, quercetin.

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