Melnikov OF, Bredun OYu, Belousova AO, Smagina TV, Zabolotna DD, Rylska OG, Peleshenko NO, Myronyuk BM, Timchenko MD, Faraon IV, Tymchenko SV
The role of lymphoepithelial structures of the upper respiratory tract in the formation of local cellular factors of innate immunity. Message 1. Participation of various formats of the lymphopharyngeal ring of Waldeyer-Pirogov in the migration of lymphocytes into the secrets of the oropharynx
Melnikov Oleg F.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Head of the Laboratory of Pathophysiology and Immunology (with a group of pathomorphology)
Doctor of Medical Sciences, Professor
E-mail: elenamelnykova@gmail.com
ORCID ID: https://orcid.org/0000-0002-1261-8647
https://www.scopus.com/authid/detail.uri?authorId=7006385903
https://www.scopus.com/authid/detail.uri?authorId=7003452154
Bredun Alexander Yu
Shupyk National Healthcare University of Ukraine (Kyiv, Ukraine)
Department of Pediatric Otorhinolaryngology, Audiology, Phoniatrics
Associate Professor, PhD.
E-mail: abredun@ukr.net
ORCID ID: http://orcid.org/0000-0002-5013-7318
Researcher ID: X-2623-2018
Belousova Alla
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Laboratory of Pathophysiology and Immunology (with a group of pathomorphology)
PhD
E-mail: amtc@kndio.kiev.ua
Smagina Tetyana V.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”
Department of Rhinology and Allergology with radiology group
Candidate of Medical Sciences
Email: amtc@kndio.kiev.ua
https://orcid.org/0000-0002-8349-8275
Zabolotna Diana D
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Head of Department of Rhinology and Allergology with a group of radiology
Doctor of Medical Sciences, Professor
E-mail: dianazab@ukr.net
ORCID ID: https://orcid.org/0000-0001-7807-8148
Rylska Oksana G.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Department of Inflammatory Diseases of ENT Organs
Senior Researcher
Email: oxanar@ua.fm
ORCID ID: https://orcid.org/0000-0003-3990-7454
Peleshenko Natalia O.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Department of Inflammatory Diseases of ENT Organs
Senior Researcher
E-mail: peleshenkon@gmail.com
Myronyuk Boris
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Department of Pediatric ENT Pathology
Senior researcher
Candidate of Medical Sciences
E-mail: amtc@kndio.kiev.ua
Timchenko Мarina D.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Senior Researcher
Laboratory of Pathophysiology and Immunology (with a group of pathomorphology)
PhD
E-mail: svtim@i.ua
https://orcid.org/0000-0002-7889-1152
Faraon Inna V
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Laboratory of Pathophysiology and Immunology (with a group of pathomorphology)
Junior Researcher
E-mail: amtc@kndio.kiev.ua
ORCID ID: 0000-0003-1010-4308
Timchenko Sergei V.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Laboratory of Pathophysiology and Immunology (with a group of pathomorphology)
Leading Researcher
Candidate of Medical Sciences
E-mail: svtim@i.ua
THE ROLE OF LYMPHOEPITHELIAL STRUCTURES OF THE UPPER RESPIRATORY TRACT IN THE FORMATION OF LOCAL CELLULAR FACTORS OF INNATE IMMUNITY.
MESSAGE 1. PARTICIPATION OF VARIOUS FORMATS OF THE LYMPHOPHARYNGEAL RING OF WALDEYER-PIROGOV IN THE MIGRATION OF LYMPHOCYTES INTO THE SECRETS OF THE OROPHARYNX
Melnikov O.F., Bredun O.Yu., Belousova A.O., Smagina T.V., Zabolotna D.D., Rylska O.G.,
Peleshenko N.O., Myronyuk B.M., Timchenko M.D., Faraon I.V., Tymchenko S.V.
State Institution «O. S. Kolomiychenko Institute of Otolaryngology
of National Academy of Medical Sciences of Ukraine»
Email: elenamelnykova@gmail.com
Abstract
The purpose of the study: to find out the role of the pharyngeal (PhT) and palatine (PT) tonsils in the influx of lymphocytes into the composition of mixed saliva.
Material and methods: The relative cellular composition of oropharyngeal secretions was studied in two groups of children with hypertrophy of pharyngeal or palatine tonsils before and 7-14 days after adenotomy, as well as in adult donors with or without PhT and PT, as well as in patients with chronic rhinosinusitis. and chronic pharyngitis with preserved or removed PhT and PT or with presence of PhT in removed PT and patients with chronic tonsillitis with presence or absence of PT. A light microscopy study of adenoid vegetations stained with hematoxylin and eosin was carried out.
Results: In children with hypertrophy of PhT, the relative content of lymphocytes in the oral fluid is significantly higher than that with an increase in PT. Adenotomy reduces the content of lymphocytes in the secretion of the oropharynx in case of excessive size of PhT and increases it in the case of growth of PT. In donors or patients with chronic rhinosinusitis, there are no significant changes in the number of lymphocytes in the composition of the oral fluid, depending on the preservation of the structures of the lymphadenoid pharyngeal ring of Waldeyer-Pirogov. With chronic pharyngitis, the number of these cells in the secretion of the oropharynx is significantly increased in the absence of PT. With chronic tonsillitis, the presence of lymphocytes in mixed saliva increases significantly in the absence of PhT. In all patients with chronic inflammatory diseases of the upper respiratory tract, the number of lymphocytes in the oral fluid exceeds that of donors.
Conclusions: In patients with chronic inflammatory processes of the upper respiratory tract, an increase in the content of lymphocytes in the cellular component of mixed saliva is observed. The pharyngeal tonsil is one of the sources of the influx of lymphocytes into the composition of mixed saliva, which has the appropriate structural components for the massive release of lymphocytes to the surface of the mucous membrane. The palatine tonsils, unlike the pharyngeal tonsils, have an inhibitory effect on the migration of lymphocytes to the oral fluid, which is clearly evident in the case of their hypertrophy in children or chronic pharyngitis in adults. In chronic tonsillitis, preservation of the palatine tonsils is not accompanied by inhibition of lymphocyte exit from the mucous membrane to the secretion of the oropharynx, which may be a consequence of the functional activity of these organs being disturbed by a pathological process localized in them.
Key words: Lymphocytes, mixed saliva, palatine tonsils, pharyngeal tonsil, chronic rhinosinusitis, chronic pharyngitis, chronic tonsillitis.
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